Biacore t200 instrument handbook, the instruction manual for operating the biacore t200 instrument biacore t200 software handbook, the instruction manual for biacore t200 control and evaluation software what to bring for your first experiment running buffer ligand molecule 2. Start the biacore t200 control software link on the desktop or in windows menu. Kinetic and affinity analysis with biaevaluation software. Twoday course covering interpretation and evaluation of kinetic and affinity data for biacore t200, biacore t100, and biacore x100 users. Ge healthcare labelfree interaction analysis biacore 8k. The specificity of a biacore analysis is determined through the nature and. Biacore x100 handbook br100810 edition ac 9 important user information important user information biacore x100 is intended for research use only and should not be used for diagnostic purposes in any clinical or in vitro procedures. Software with flat interface for maximum overview biacore 8k software exhibits a flat interface that provides overview and intuitive, rapid operation.
The new t200 control software is newly designed and not compatible with biamethodwriter. Resources shared resources for duke human vaccine institute. Biacore 3000 primed to perfection for charaterization. Biacore t100 software version 2 university of oxford. Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Data file 29050011 aa labelfree interaction analysis. Biacore experiment performed using aminecoupling chemistry. When you are interested in ligand fishing, molecular recognition, biams or other advanced applications. Biacore t200 software handbookdescribes how to use the software for creating and editing methods, running experiments and evaluating results. Biacore, t200 the department of physiology and biophysics. Biacore t200 instrument handbook guides you through operation and maintenance of the biacore t200 system. Biacore maintenance kits are convenient kits for proper instrument maintenance. Instrumentspecific and general methodology handbooks can be accessed from the handbooks download page. Biacore t200 software handbook, the instruction manual for biacore t200 control and evaluation software what to bring for your first experiment running buffer ligand molecule 250 gml in immobilization buffer, higher concentration if scouting conditions for immobilization.
Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. Biacore t200 software handbook 28976878 edition aa 7 introduction 1 1 introduction biacore t200 is a high performance sy stem for analysis of biomolecular interactions, based on ge healthcares surface plasmon resonance spr technology. The handbook also includes a system description, safety instructions, specifications and a brief description of the spr principle. T200 software handbook full manual for instrument operation and data analysis using the control and evaulation software platforms. Compare the reichert sr7500 dual channel system to the.
With a single click, the compact designed equipment offers realtime run control and. Biacore t200 getting started biacore t200 getting started introduction this getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and biacore t200 evaluation software. Software associated documents such as software notes are available for download via the relevant software folders below. Biacore sensorgram the systems also provide sensitive, accurate concentration measurements. Ge biacore t200 getting started guide ligand analyte.
For company and institution core facilities, proteomics groups, cancer research facilities. This getting started handbook is designed as a selfstudy guide to introduce you to the basic operations of biacoretm t200, biacore t200 control software and. Biacore spr technology cytiva, formerly ge healthcare. The handbook, together with the associated reagent kit, will take you through.
Biacore t200 software offers a range of tools for confident and reliable kinetic analyses. Use this handbook together with the specific user documentation for your biacore system, which will describe how to implement the assay principles in the instrument and system software. The biamethodwriter is a program to write the biacore automation methods in a more interactive way. Reichert sr7500dc biacore x100 biacore t200 number of channels 22 4 injection of lysates, cells, particulates vyes no no diy fluidics vyes no no autosampler capacity vmax. Biacore t200 evaluation software enables kinetic and affinity evaluations of. This is based on the ability of the biomolecule of interest to interact with a specific binding partner, and may therefore be more informative than generic measurement. Biacore t200 for high sensitivity, labelfree interaction analysis. Biacore sensor surface handbook br100571 ac 11 introduction 1 1. Kinetic and affinity analysis with system specific evaluation software.
These analyses can be performed using a multicycle approach many samples against one ligand or when different ligands are immobilized, or alternatively, using singlecycle kinetics fast runs without regeneration. Biacore 8k provides highquality data at physiologically relevant temperatures. Includes detailed information on data acquisition modes manual runs, methods, and application wizards and data analysis techniques including. Ge healthcare labelfree interaction analysis biacore t200.
Cross new frontiers in labelfree interaction analysis with biacore t200, ge healthcare, 28979412, edition aa 2010 data file. Softwareassociated documents such as software notes are available for download via the relevant software folders below. This edition of the biacore x100 handbook applies to systems running software version 2. Software wizard templates to help speed up assay optimization and routine analyses. Biacore t200 software handbook 28976878 edition aa. Biacore t200 software handbook describes how to use the software for creating and editing methods, running experiments and evaluating results.
This handbook describes biacore t100 control software and evaluation software, version 2. It works for the control software with the biacore 20003000. Labelfree interaction analysis ge healthcare fig 1. This video show you how to startup and prepare a biacore t200 system for use. Biacore assay handbook ge healthcare life sciences. The interaction being studied occurs on the surface of the chip. Legal information regarding software licensing and details for acquiring additional licenses. This program uses popup menus and dialog boxes to fill in the different parts. Any functionality that is added in optional addon modules is described in separate documentation. Biacore t200, ge healthcare, 28979415, edition ab 20. Biacore t200 cytiva, formerly ge healthcare life sciences. This video guides you through the evaluation workflow of a calibrationfree concentration assay cfca in the biacore t200 evaluation software. Biacore t200 instrument handbookdescribes the instrumentation in the biacore t200 system, with instructions for operation, maintenance and troubleshooting.
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